ccl22 recruits cd4-全讯担保网

purpose: the aim of this study was to explore the presence of the chemokines ccl22 and ccl17 in malignant pleural effusion, and the chemoattractant activity of these chemokines on cd4-positive cd25-positive foxp3-positive reg ulatory tcells infiltrating into the pleural space. experimental design: the concentrations of ccl22 and ccl17 in both pleural effusions and sera from 33 patients with lung cancer were determined. flow cytometry was done to determine t lymphocyte subsets in cell pellets of pleural effusion. pleural cells were analyzed for the expres-sion of ccl22 and ccl17. the chemoattractant activity of ccl22 for regulatory tcells in vitro and in vivo was also observed. results: the concentration of ccl22 in malignant pleural effusion was significantly higher than that in the corresponding serum. pleural fluid from lung cancer patients was chemotactic for reg-ulatorytcells, and this activity was partly blocked by an anti-ccl22, but not by an anti-ccl17 antibody. intrapleural administration of ccl22 0f patients produced a marked progressive influx of reg ulatory t cells into pleural space. conclusions: compared with serum, ccl22 seemed to be increased in malignant pleural effu-sion, and could directly induce reg ulatory t cell infiltration into the pleural space in patients with malignant effusion.