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the aim of this study was to investigate the presence of epithelial neutrophil-activating peptide (ena)-78 in pleural effusions, as well as the chemoattractant activity of pleural ena-78 0n neutrophils. pleural effusion and serum samples were collected from 75 patients who presented to the respiratory institute (19 with malignant pleural effusion, 21 with tuberculous pleural effusion, 18 with infectious pleural effusion and 17 with transudative pleural effusion). the concentrations of ena-78, myeloperoxidase and neutrophil elastase were determined, and the chemoattractant activity of ena-78 for neutrophils both in vitro and in vivo was also observed. the concentrations of ena-78, myeloperoxidase and neutrophil elastase in infectious pleural effusion were significantly higher than those in malignant, tuberculous and transudative groups, respectively (all p<0.01). infectious pleural fluid was chemotactic for neutrophils in vitro and anti-ena-78 antibody could partly inhibit these chemotactic effects. intrapleural administration of ena-78 produced a marked progressive influx of neutrophils into pleural space. compared with noninfectious pleural effusion, ena-78 appeared to be increased in infectious pleural effusion. our data suggested that ena-78 was able to induce neutrophil infiltration into pleural space and might be responsible for pleural neutrophil degranulation.

allergic asthma is characterized by airway hyper-responsiveness and chronic mucosal inflammation mediated by cd4 th2 lymphocytes. regulatory cd4 cd25 t cells are important components of the homeostasis of the immune system, as impaired cd4 cd25 t cell activity can cause autoimmune diseases and allergy. the mechanism of suppression by cd4 cd25 t cells remains controversial; different in vivo and in vitro studies raise possible roles for the immunosuppressive cytokines interleukin-10 and transforming growth factor-β, forkhead transcription factor foxp3, glucocorticoid-induced tumor necrosis factor receptor, cytotoxic lymphocyte associated antigen-4, 4-1bb costimulator receptor, a cd4-related molecule lag-3, and neuropilin-1. current data suggest that th2 responses to allergens are normally suppressed by cd4 cd25 t cells. suppression by cd4 cd25 t cells is decreased in allergic individuals. furthermore, cd4 cd25 t cells play a key role in regulating airway eosinophilic inflammation. the immunomodulatory properties of cd4 cd25 t cells do extend to th2 responses, most notably by limiting the development of a proinflammatory cd4 th2 phenotype characterized by reduced cytokine production. an understanding of the roles of cd4 cd25 t cells in vivo could provide better insight into the design of novel approaches to modulate the chronic airway inflammatory reaction evident in bronchial asthma.

conventional tests are not always helpful in making a diagnosis of tuberculous pleurisy. many studies have investigated the usefulness of interferon (ifn)-γ measurements in pleural fluid for the early diagnosis of tuberculous pleurisy. we conducted a metaanalysis to determine the accuracy of ifn-γ measurements in the diagnosis of tuberculous pleurisy. methods: after a systematic review of english-language studies, sensitivity, specificity, and other measures of accuracy of ifn-γ concentrations in the diagnosis of pleural effusion were pooled using random-effects models. summary receiver operating characteristic curves were used to summarize overall test performance. results: twenty-two studies met our inclusion criteria. the summary estimates for ifn-γ in the diagnosis of tuberculous pleurisy in the studies included were as follows: sensitivity, 0.89 (95% confidence interval [ci], 0.87 to 0.91); specificity, 0.97 (95% ci, 0.96 to 0.98); positive likelihood ratio, 23.45 (95% ci, 17.31 to 31.78); negative likelihood ratio, 0.11 (95% ci, 0.07 to 0.16); and diagnostic odds ratio, 272.7 (95% ci, 147.5 to 504.2). conclusions: ifn-γ determination is a sensitive and specific test for the diagnosis of tuberculous pleurisy. the measurement of ifn-γ levels in pleural effusions is thus likely to be a useful tool for diagnosing tuberculous pleurisy. the results of ifn-γ assays should be interpreted in parallel with clinical findings and the results of conventional tests. (chest 2007; 131:1133–1141)

study objective: to investigate the effects of segmental allergen challenge on the concentration of soluble cd86 (scd86) in bal fluids in patients with allergic asthma. methods: bal fluid and peripheral blood were collected at baseline, 24h after segmental saline solution or allergen challenge by fiberoptic bronchoscopy and venepuncture, respectively, from 10 patients with allergic asthma. total and differential cell counts in bal fluid were performed, and scd86 levels in both bal fluid and serum were measured by enzyme-linked immunosorbent assay. results: in allergic asthmatics, there was no significant increase in bal scd86 concentrations after saline solution challenge (median, 2.0iu/l; 25th to 75th percentiles, 0 to 3.4) compared with baseline control subjects (median, 1.2iu/l; 25th to 75th percentiles, 0 to 3.6iu/ml; p 0.735); however, scd86 concentrations were significantly elevated after allergen challenge (median, 8.1iu/l; 25th to 75th percentiles, 4.4 to 17.0iu/ml; p<0.001). the concentrations of scd86 in bal fluid after allergen challenge exceeded levels that could be accounted for passive transudation from the circulation, based on the magnitude of increases in bal albumin concentrations. conclusions: these data indicate that allergen challenge results in a significant local accumulation of scd86 within the airways, and that the local release of scd86 may play a role in allergen-induced inflammatory processes in the asthmatic airways.

il-17–producing cd4 t (th17) cells have been found to be increased in some human cancers; however, the possible implication of th17 cells in regulating antitumor responses in malignant pleural effusion (mpe) remains to be elucidated. in the current study, distribution and phenotypic features of th17 cells in both mpe and peripheral blood from patients with lung cancer were determined by flow cytometry or double immunofluorescence staining. the impacts of cytokines on th17 cell generation and differentiation were explored. the chemoattractant activity of chemokines ccl20 and ccl22 for th17 cells in vitro was also observed. it was found that the increased th17 cells could be found in mpe compared with blood. the in vitro experiments showed that il-1b, il-6, il-23, or their various combinations could promote th17 cell generation and differentiation from naive cd4 t cells. mpe was chemotactic for th17 cells, and this activity was partly blocked by anti-ccl20 and/or ccl22 abs. our data also showed that the accumulation of th17 cells in mpe predicted improved patient survival. it could be concluded that the overrepresentation of th17 cells in mpe might be due to th17 cell differentiation and expansion stimulated by pleural proinflammatory cytokines and to recruitment of th17 cells from peripheral blood induced by pleural chemokines ccl20 and ccl22. furthermore, the accumulation of th17 cells in mpe predicted improved patient survival. these data provide the basis for developing immune-boosting strategies based on ridding the cancer patient of this cell population. the journal of immunology,