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期刊论文
analysis of oligomannuronic acids and oligoguluronic acids by high-performance anion-exchange chromatography and electrospray ionization mass spectrometry
journal of chromatography a, 884(2000)105-111，-0001，（）：
oligomannuronic acids and oligoguluronic acids were prepared by enzymatic hydrolysis of alginate with alginate lyases. the oligosaccharides generated up to degree of polymerization 16 were characterized by high-performance anion-exchange chromatography (hpaec) with pulsed amperometric detection (pad) and electrospray ionization mass spectrometry (esi-ms). acetate buffer linear gradients were used as mobile phases for separations of oligosaccharides. esi-ms and hpaec-pad are very effective for the analysis and characterization of anionic oligosaccharides.
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