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bmal1过表达促进结直肠癌caco-2细胞增殖与迁移的作用研究
首发时间：2024-06-06
摘要：结直肠癌（colorectal cancer, crc）是全球范围内的主要恶性肿瘤之一，其发病率和死亡率呈上升趋势。本研究聚焦于脑和肌肉芳基烃受体核转运体样1（brain and muscle arnt-like-1，bmal1），一个在调控生物节律中起关键作用的基因，探讨其在结直肠癌中的潜在作用。通过构建tet-on诱导表达系统，我们在caco-2细胞系中实现了bmal1的过表达，并评估了其对细胞增殖和迁移的影响。实验结果表明，与对照组相比，bmal1过表达组的细胞增殖率显著提高，edu 细胞数量增多，细胞周期相关基因表达上调。此外，bmal1过表达还显著增强了caco-2细胞的迁移能力，这一点通过划痕实验得到了证实。western blot分析进一步揭示了bmal1过表达可能通过调节rho相关激酶rock1、粘附连接蛋白β-catenin、钙粘蛋白e-cadherin的表达和粘着斑激酶fak的磷酸化来促进细胞迁移。本研究的发现为理解bmal1在结直肠癌发展中的作用提供了新视角。
关键词： bmal1 结直肠癌 细胞增殖 细胞迁移 caco-2细胞
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study on the role of bmal1 overexpression in promoting proliferation and migration of caco-2 cells
abstract：colorectal cancer (crc) is one of the leading malignant tumors globally, with increasing incidence and mortality rates. this study focuses on brain and muscle arnt-like-1 (bmal1), a key gene in regulating biological rhythms, to explore its potential role in crc. by constructing a tet-on inducible expression system, we achieved bmal1 overexpression in caco-2 cell lines and assessed its impact on cell proliferation and migration. experimental results showed that, compared to the control group, bmal1 overexpression significantly increased cell proliferation rate, edu cell count, and the expression of cell cycle-related genes. additionally, bmal1 overexpression markedly enhanced caco-2 cell migration, as evidenced by scratch assays. western blot analysis further revealed that bmal1 overexpression may promote cell migration by regulating the expression of rho-associated kinase rock1, adhesion protein β-catenin, cadherin e-cadherin, and the phosphorylation of focal adhesion kinase fak. these findings provide new insights into the role of bmal1 in crc development.
keywords： bmal1 colorectal cancer cell proliferation cell migration caco-2 cells
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bmal1过表达促进结直肠癌caco-2细胞增殖与迁移的作用研究
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