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环境标准限值浓度 砷致斑马鱼心血管发 育毒性
首发时间：2024-05-16
摘要：（砷是一种对人类心血管健康构成严重威胁的有害物质。为探讨环境浓度砷对心血管发育的影响，本研究选取"环境标准限值浓度"砷（5、10、50、150 和 500 μg/l）对斑马鱼胚胎进行染毒，暴露时间为4-120 hpf。评估心血管发育的形态学和功能学指标，包括心脏畸形率、静脉窦-动脉球间距、心率、心室收缩分数、血流动力学。使用定量逆转录聚合酶链反应（qrt-polymerase chain reaction, qrt-pcr，简称qpcr）检测斑马鱼心血管发育相关基因的表达情况。结果显示，"环境标准限值浓度"砷可影响斑马鱼心血管发育，具体表现为心率减慢、心室收缩分数降低、血流减缓以及心血管发育相关基因表达异常。
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\'environmental standard limit concentration arsenic\' induced cardiovascular developmental toxicity in zebrafish
abstract：arsenic is a harmful substance that poses a serious threat to human cardiovascular health. to explore the effects of environmental concentrations of arsenic on cardiovascular development, this study exposed zebrafish embryos to \'environmental standard limit concentrations\' arsenic (5, 10, 50, 150, and 500 μg/l) for a duration of 4 to 120 hpf (hours post-fertilization). morphological and functional indicators of cardiovascular development in zebrafish were evaluated, including heart malformation rate, sinus venosus-bulbus arteriosus distance, heart rate, ventricular fractional shortening, and hemodynamics. the expression of genes related to cardiovascular development in zebrafish was detected using quantitative reverse transcription-polymerase chain reaction (qrt-pcr, or qpcr for short). the results showed that \'environmental standard limit concentrations\' arsenic could affect cardiovascular development in zebrafish, specifically manifesting as a slower heart rate, decreased ventricular fractional shortening, slower blood flow, and abnormal expression of genes related to cardiovascular development.
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环境标准限值浓度 砷致斑马鱼心血管发 育毒性
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