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brd9在vd/vdr影响糖尿病中的作用及机制探究
首发时间：2024-04-01
摘要：目的：探究brd9蛋白在vd/vdr缓解糖尿病症状中的影响。方法：60%高脂饮食喂养6周后腹腔注射stz建立2型糖尿病模型。分别以10 mg/kg ibrd9或5 μg/kg 1,25(oh)2d3进行干预。6周后检测各组小鼠糖代谢指标空腹血糖（fbg、胰岛素含量ins、糖耐量ogtt)；elisa检测血清il-1β、tnf-α、ifn-γ三种炎症因子的分泌水平；western blot实验分析各组小鼠胰腺组织炎症情况；he染色观察胰腺组织病理变化。结果：t2dm小鼠糖代谢明显异常，表现为空腹血糖值增高，糖耐量受损，胰岛素分泌减少，并且炎症因子释放水平和炎症相关蛋白表达量明显增多（p＜0.0001，p＜0.001)，he染色发现胰岛体积减小，胰岛内细胞分布不均；药物干预后血糖水平明显降低，同时空腹胰岛素含量显著增加，炎症相关蛋白和炎症因子表达有所减少（p＜0.05，p＜0.001)，胰岛形态趋向正常；ibrd9-vd联用时小鼠炎症相关蛋白及炎症因子表达降低更显著（p＜0.05，p＜0.01)，同时glut2和pdx1表达增多明显（p＜0.01)。结论：t2dm发生时炎症因子分泌增加，胰腺 β 细胞功能受损，小鼠糖代谢紊乱；抑制brd9蛋白能够提高t2dm小鼠机体糖代谢能力，降低机体炎症水平，改善 β 细胞障碍；brd9可抑制vd和vdr，发挥其生物学效应。???
关键词： 炎症
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the role and mechanism of brd9 in vd/vdr in diabetes mellitus
abstract：objective: to explore the influence of brd9 on alleviating diabetes symptoms in vd/vdr. methods: a 60% high-fat diet was fed for 6 weeks followed by intraperitoneal injection of stz to establish a type 2 diabetes model. interventions were administered with either 10 mg/kg ibrd9 or 5 μg/kg 1,25(oh)2d3. after 6 weeks, various metabolic indicators (fasting blood glucose, insulin content, glucose tolerance ) were measured in each group of mice; elisa was used to detect serum levels of inflammatory factors il-1β, tnf-α, and ifn-γ secretion levels; western blot was used to analyze the inflammation situation in pancreatic tissue of each group of mice; he staining was performed to observe pathological changes in pancreatic tissue. results: t2dm mice showed significant abnormalities in glucose metabolism, characterized by increased fasting blood glucose levels, impaired glucose tolerance, decreased insulin secretion, and significantly increased levels of inflammatory cytokines and expression of inflammatory-related proteins (p <0.0001, p <0.001). he staining revealed reduced islet volume and uneven distribution of cells within the islets. after drug intervention, blood glucose levels decreased significantly, while fasting insulin levels increased significantly, and expression of inflammatory-related proteins and inflammatory factors decreased (p <0.05, p <0.001), with islet morphology trending towards normal. when ibrd9 and vd were used in combination, the expression of inflammatory-related proteins and inflammatory factors in mice decreased more significantly (p <0.05, p <0.01), while the expression of glut2 and pdx1 increased significantly (p <0.01). conclusion: increased secretion of inflammatory factors occurs when t2dm develops, leading to impaired pancreatic β-cell function and disrupted glucose metabolism in mice. inhibiting the brd9 protein can improve the glucose metabolism capacity of t2dm mice, reduce inflammatory levels in the body, and improve β-cell dysfunction. brd9 can inhibit the binding of vd and vdr, exerting its biological effects.
keywords： inflammation
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brd9在vd/vdr影响糖尿病中的作用及机制探究
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