提示
中性粒细胞耗竭影响肠道微生物多样性：一项宏基因组学层面的研究
首发时间：2024-03-18
摘要：目的：探究中性粒细胞对肠道健康的影响。方法：通过构建小鼠中性粒细胞耗竭模型，采样小鼠肠内容物进行宏基因组测序分析。结果：小鼠中性粒细胞耗竭模型构建成功，在门水平上，中性粒细胞耗竭使梭杆菌门和迷踪菌门升高；在纲水平上，中性粒细胞耗竭使梭杆菌纲、迷踪菌纲、噬几丁质菌纲、甲烷微菌纲、甲烷球菌纲、亚硝酸球藻纲、异常球菌纲升高；在属水平上，中性粒细胞耗竭使梭杆菌属、迷踪菌属、乳球菌属、漫游菌属、黄杆菌属、甲烷八叠球菌属升高；在种水平上，中性粒细胞耗竭使艰难梭菌、化脓性链球菌、大肠杆菌和金黄色葡萄球菌升高。结论：中性粒细胞可以抑制梭杆菌门-梭杆菌纲-梭杆菌目-梭杆菌科-梭菌属-艰难梭菌、迷踪菌门-迷踪菌纲-迷踪菌目-迷踪菌科-迷踪菌属-迷踪菌、化脓性链球菌、大肠杆菌和金黄色葡萄球菌这些致病菌从而维持肠道稳态。
for information in english, please click here
the impact of neutrophil depletion on gut microbial diversity: a metagenomic perspective
abstract： purpose: to explore the impact of neutrophils on gut health. methods: the mouse neutrophil depletion model was established, and the intestinal contents of mice were sampled for metagenomic sequencing. results: the mouse neutrophil depletion model was established successfully. at the phylum level, neutrophil depletion increased fusobacteria and elusimicrobia. at the class level, neutrophil depletion increased fusobacteriia、endomicrobia、chitinophagia、methanomicrobia、methanococci、nitrososphaeria and deinococci. at the genus level, neutrophil depletion increased fusobacterium、endomicrobium、lactococcus、vagococcus、tenacibaculum and methanosarcina. at the species level, neutrophil depletion incerased fusobacterium varium、streptococcus pyogenes、escherichia coli and staphylococcus aureus. conclusion: neutrophils can inhibit fusobacteria-fusobacteriia-fusobacteriales-fusobacteriaceae-fusobacterium-fusobacterium varium、elusimicrobia-endomicrobia-endomicrobiales-endomicrobiaceae-endomicrobium-endomicrobium proavitum、streptococcus pyogenes、escherichia coli and staphylococcus aureus to maintain intestinal homeostasis.
keywords： neutrophil gut microbiota metagenome
基金：
论文图表：
引用
导出参考文献
no.****
动态公开评议
共计0人参与
勘误表
中性粒细胞耗竭影响肠道微生物多样性：一项宏基因组学层面的研究
评论
全部评论